Hotspot

ndhC-trnV-UAC

LSC · 53474-55016 · 1543 bp

Back to Commelina

Pi 0.0179

MarkerSeek score 64.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1543
variable_sites: 40
pi: 0.0179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CAGATTCGTGAAGCAGAAACA	GAGAAGGTCTACGGTTCGAG	42.9	55.0	56.8	57.2	1519-1599 bp	1.0	62.2
ndhC-trnV-UAC_p2	CAGATTCGTGAAGCAGAAACA	CGAGAAGGTCTACGGTTCGA	42.9	55.0	56.8	58.9	1520-1600 bp	1.0	61.5
ndhC-trnV-UAC_p3	TCAGATTCGTGAAGCAGAAACA	GAGAAGGTCTACGGTTCGAG	40.9	55.0	58.3	57.2	1520-1600 bp	1.0	61.2
ndhC-trnV-UAC_p4	TCAGATTCGTGAAGCAGAAACA	CGAGAAGGTCTACGGTTCGA	40.9	55.0	58.3	58.9	1521-1601 bp	1.0	60.5
ndhC-trnV-UAC_p5	TCAGATTCGTGAAGCAGAAAC	GAGAAGGTCTACGGTTCGAG	42.9	55.0	56.6	57.2	1520-1600 bp	1.0	60.0