Hotspot

pafI-trnS-GGA

LSC · 47103-47693 · 591 bp

Back to Commelina

Pi 0.0141

MarkerSeek score 63.80

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 591
variable_sites: 12
pi: 0.0141
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	TTTTCTCCGGAAGTAGTCGG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.0	58.2	777-809 bp	1.0	72.1
pafI-trnS-GGA_p2	TTTTCTCCGGAAGTAGTCGG	TTCCAATGCTACGCCTTCAA	50.0	45.0	57.0	57.8	727-759 bp	1.0	71.9
pafI-trnS-GGA_p3	TTTTCTCCGGAAGTAGTCGG	AGTTCCAATGCTACGCCTTC	50.0	50.0	57.0	58.3	729-761 bp	1.0	71.3
pafI-trnS-GGA_p4	TTTTTCTCCGGAAGTAGTCGG	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.7	58.2	778-810 bp	1.0	69.8
pafI-trnS-GGA_p5	TTTTTCTCCGGAAGTAGTCGG	TTCCAATGCTACGCCTTCAA	47.6	45.0	57.7	57.8	728-760 bp	1.0	69.6