Hotspot

trnE-UUC-trnT-GGU

LSC · 33382-34009 · 628 bp

Back to Commelina

Pi 0.0311

MarkerSeek score 71.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 628
variable_sites: 24
pi: 0.0311
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	ACGACTTACGCCTTACCATG	50.0	50.0	57.8	57.7	680-727 bp	1.0	83.1
trnE-UUC-trnT-GGU_p2	CTTCTTTTCGTGGTACCCCC	ACGACTTACGCCTTACCATG	55.0	50.0	58.2	57.7	717-764 bp	1.0	82.8
trnE-UUC-trnT-GGU_p3	TCCGCTTCTTTTCGTGGTAC	ACGACTTACGCCTTACCATG	50.0	50.0	58.2	57.7	721-768 bp	1.0	82.6
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	ACGACTTACGCCTTACCATG	50.0	50.0	57.7	57.7	658-705 bp	1.0	82.3
trnE-UUC-trnT-GGU_p5	TCCACGATCCGCTTCTTTTC	ACGACTTACGCCTTACCATG	50.0	50.0	58.3	57.7	728-775 bp	1.0	82.0