Hotspot

atpF-atpH

LSC · 14474-14981 · 508 bp

Back to Commelina

Pi 0.0132

MarkerSeek score 66.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 508
variable_sites: 10
pi: 0.0132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	ACAATTTACGGGTTGGTCGT	50.0	45.0	57.9	57.7	634-641 bp	1.0	79.2
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AATTTACGGGTTGGTCGTGG	50.0	50.0	57.9	58.5	632-639 bp	1.0	77.5
atpF-atpH_p3	CAGTGGCCCAAGGAAATGAA	ACAATTTACGGGTTGGTCGT	50.0	45.0	58.4	57.7	606-613 bp	1.0	77.3
atpF-atpH_p4	AGTGGCCCAAGGAAATGAAA	ACAATTTACGGGTTGGTCGT	45.0	45.0	57.6	57.7	605-612 bp	1.0	77.1
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CAATTTACGGGTTGGTCGTG	50.0	50.0	57.9	57.4	633-640 bp	1.0	76.5