Hotspot

trnS-GCU-trnG-UCC

LSC · 8861-10390 · 1530 bp

Back to Commelina

Pi 0.0196

MarkerSeek score 67.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1530
variable_sites: 43
pi: 0.0196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	1604-1808 bp	1.0	56.5
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	1607-1811 bp	1.0	55.4
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	1606-1810 bp	1.0	55.4
trnS-GCU-trnG-UCC_p4	AGTAAGCGGAGAGAAAGGGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.8	55.5	1658-1862 bp	1.0	54.8
trnS-GCU-trnG-UCC_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	1605-1809 bp	1.0	54.6