Hotspot

trnK-UUU-rps16

LSC · 4337-4880 · 544 bp

Back to Commelina

Pi 0.0233
MarkerSeek score 62.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
544
variable_sites
19
pi
0.0233
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGTGGTCTTATAGACTCCGC AGAAGGCAGAAATTTTTAAAGA 55.0 27.3 57.6 52.2 700-710 bp 1.0 42.4
trnK-UUU-rps16_p2 TGGTCTTATAGACTCCGCCA AGAAGGCAGAAATTTTTAAAGA 50.0 27.3 57.6 52.2 698-708 bp 1.0 42.4
trnK-UUU-rps16_p3 CGTGGTCTTATAGACTCCGC AAGAAGGCAGAAATTTTTAAAGA 55.0 26.1 57.6 53.1 701-711 bp 1.0 42.4
trnK-UUU-rps16_p4 CGTGGTCTTATAGACTCCGC AGAAGGCAGAAATTTTTAAAGAA 55.0 26.1 57.6 53.1 700-710 bp 1.0 42.4
trnK-UUU-rps16_p5 TGGTCTTATAGACTCCGCCA AGAAGGCAGAAATTTTTAAAGAA 50.0 26.1 57.6 53.1 698-708 bp 1.0 42.4