Hotspot

ndhE

SSC · 122064-122369 · 306 bp

Back to Combretum

Pi 0.00327

MarkerSeek score 41.10

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 1
pi: 0.00327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TACATCTGTTGGGCATGCTC	ACGCGATTATTTGGACGACA	50.0	45.0	58.0	58.0	1059-1066 bp	1.0	86.4
ndhE_p2	ACCCATGCTTCGAGTTGTTT	ACGCGATTATTTGGACGACA	45.0	45.0	58.0	58.0	1218-1225 bp	1.0	85.8
ndhE_p3	CGACACAATCCTCGGTTCTT	ACGCGATTATTTGGACGACA	50.0	45.0	57.9	58.0	1132-1139 bp	1.0	85.8
ndhE_p4	GCTTCGAGTTGTTTCATGCC	ACGCGATTATTTGGACGACA	50.0	45.0	58.0	58.0	1212-1219 bp	1.0	85.7
ndhE_p5	GCTTTACATCCATCCCAGGG	ACGCGATTATTTGGACGACA	55.0	45.0	58.0	58.0	1098 bp	0.5	59.9