Hotspot

JXY33_pgt034-JXY33_pgt035

IRa · 139843-139910 · 68 bp

Back to Combretum

Pi 0.0441

MarkerSeek score 77.90

Primer available yes

Label JXY33_pgt034-JXY33_pgt035

Feature Metadata

label_name: JXY33_pgt034-JXY33_pgt035
feature_type: igs
region: IRa
length_bp: 68
variable_sites: 3
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
JXY33_pgt034-JXY33_pgt035_p1	CATTACCGCCCGGACAATTA	TGATTTACTTCACGGGCGAG	50.0	50.0	58.0	58.0	204 bp	1.0	80.8
JXY33_pgt034-JXY33_pgt035_p2	CATTACCGCCCGGACAATTA	AGGTCTCTGGTTCAAGTCCA	50.0	50.0	58.0	57.9	186 bp	1.0	80.1
JXY33_pgt034-JXY33_pgt035_p3	ATTACCGCCCGGACAATTAG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.8	58.0	203 bp	1.0	79.1
JXY33_pgt034-JXY33_pgt035_p4	TACTATCATTACCGCCCGGA	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	210 bp	1.0	78.8
JXY33_pgt034-JXY33_pgt035_p5	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	50.0	50.0	57.7	58.0	165 bp	1.0	78.7