Hotspot

rps15-JXY33_pgp007

SSC · 127889-133001 · 5113 bp

Back to Combretum

Pi 0.0239
MarkerSeek score 62.60
Primer available yes
Label rps15-JXY33_pgp007

Feature Metadata

label_name
rps15-JXY33_pgp007
feature_type
igs
region
SSC
length_bp
5113
variable_sites
114
pi
0.0239
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-JXY33_pgp007_p1 TTTTGATCCTTCCCCGATGG GGGACGAAACAGGAACAAGA 50.0 50.0 57.6 57.8 893 bp 0.5 54.5
rps15-JXY33_pgp007_p2 TTTTGATCCTTCCCCGATGG ACGAAACAGGAACAAGAGGG 50.0 50.0 57.6 57.7 890 bp 0.5 54.5
rps15-JXY33_pgp007_p3 TTTTGATCCTTCCCCGATGG GGAACAAGAGGGATTCACCG 50.0 55.0 57.6 58.3 882 bp 0.5 54.4
rps15-JXY33_pgp007_p4 TTTTGATCCTTCCCCGATGG GAAAGGAAGGGGACGAAACA 50.0 50.0 57.6 57.7 902 bp 0.5 54.4
rps15-JXY33_pgp007_p5 TTTTGATCCTTCCCCGATGG AAAGGAAGGGGACGAAACAG 50.0 50.0 57.6 57.7 901 bp 0.5 54.3