Hotspot

ndhE-ndhG

SSC · 122370-122619 · 250 bp

Back to Combretum

Pi 0.237

MarkerSeek score 77.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 250
variable_sites: 53
pi: 0.237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	ATAGCCAACCCAATAGCTGC	ACGCGATTATTTGGACGACA	50.0	45.0	58.0	58.0	634-659 bp	1.0	89.9
ndhE-ndhG_p2	TACATCTGTTGGGCATGCTC	ACGCGATTATTTGGACGACA	50.0	45.0	58.0	58.0	1059-1066 bp	1.0	86.4
ndhE-ndhG_p3	CGACACAATCCTCGGTTCTT	ACGCGATTATTTGGACGACA	50.0	45.0	57.9	58.0	1132-1139 bp	1.0	85.8
ndhE-ndhG_p4	GTTGGACAGGCAGATTCACA	ACGCGATTATTTGGACGACA	50.0	45.0	58.1	58.0	1163-1170 bp	1.0	85.4
ndhE-ndhG_p5	GCTTTACATCCATCCCAGGG	ACGCGATTATTTGGACGACA	55.0	45.0	58.0	58.0	1098 bp	0.5	59.9