Hotspot

ndhF-rpl32

SSC · 117207-117845 · 639 bp

Back to Combretum

Pi 0.219

MarkerSeek score 85.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 639
variable_sites: 132
pi: 0.219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCGAAGATTCTTTGTTGCC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1357-1722 bp	1.0	89.9
ndhF-rpl32_p2	GGCGAAGATTCTTTGTTGCC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1358-1723 bp	1.0	89.9
ndhF-rpl32_p3	ACATAGGAACCGGAAGTGGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1306-1671 bp	1.0	89.7
ndhF-rpl32_p4	TAAGCCAAAATCCCCTACGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1812-2177 bp	1.0	87.7
ndhF-rpl32_p5	TAAGCCAAAATCCCCTACGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1813-2178 bp	1.0	87.6