Hotspot

JXY33_pgt027-JXY33_pgt028

IRb · 107915-107982 · 68 bp

Back to Combretum

Pi 0.0441

MarkerSeek score 77.90

Primer available yes

Label JXY33_pgt027-JXY33_pgt028

Feature Metadata

label_name: JXY33_pgt027-JXY33_pgt028
feature_type: igs
region: IRb
length_bp: 68
variable_sites: 3
pi: 0.0441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
JXY33_pgt027-JXY33_pgt028_p1	TGATTTACTTCACGGGCGAG	CATTACCGCCCGGACAATTA	50.0	50.0	58.0	58.0	204 bp	1.0	80.8
JXY33_pgt027-JXY33_pgt028_p2	AGGTCTCTGGTTCAAGTCCA	CATTACCGCCCGGACAATTA	50.0	50.0	57.9	58.0	186 bp	1.0	80.1
JXY33_pgt027-JXY33_pgt028_p3	TGATTTACTTCACGGGCGAG	ATTACCGCCCGGACAATTAG	50.0	50.0	58.0	57.8	203 bp	1.0	79.1
JXY33_pgt027-JXY33_pgt028_p4	TGATTTACTTCACGGGCGAG	TACTATCATTACCGCCCGGA	50.0	50.0	58.0	57.7	210 bp	1.0	78.8
JXY33_pgt027-JXY33_pgt028_p5	TGATTTACTTCACGGGCGAG	CAACGACCCAATTGCAAGAG	50.0	50.0	58.0	57.7	165 bp	1.0	78.7