Hotspot

JXY33_pgt021-JXY33_pgt022

LSC · 70254-70455 · 202 bp

Back to Combretum

Pi 0.0982

MarkerSeek score 85.70

Primer available yes

Label JXY33_pgt021-JXY33_pgt022

Feature Metadata

label_name: JXY33_pgt021-JXY33_pgt022
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 16
pi: 0.0982
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
JXY33_pgt021-JXY33_pgt022_p1	ACCTACGACATCGGGTTTTG	GGTGCCACGGAAAGATATGT	50.0	50.0	57.9	58.0	739-812 bp	1.0	85.1
JXY33_pgt021-JXY33_pgt022_p2	CGGTTTTCTACTAGCAGCGT	GGTGCCACGGAAAGATATGT	50.0	50.0	58.0	58.0	1241 bp	0.5	59.6
JXY33_pgt021-JXY33_pgt022_p3	CGGTTTTCTACTAGCAGCGT	ATCTTCTTCACCCCTTCCCA	50.0	50.0	58.0	57.9	1370 bp	0.5	59.5
JXY33_pgt021-JXY33_pgt022_p4	CGGTTTTCTACTAGCAGCGT	TCTTCTTCACCCCTTCCCAT	50.0	50.0	58.0	57.9	1369 bp	0.5	59.5
JXY33_pgt021-JXY33_pgt022_p5	CGGTTTTCTACTAGCAGCGT	TTTACCCTTGGCCATGAACC	50.0	50.0	58.0	58.1	1762 bp	0.5	59.4