Hotspot

psbE-petL

LSC · 68370-69673 · 1304 bp

Back to Combretum

Pi 0.0338

MarkerSeek score 68.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1304
variable_sites: 43
pi: 0.0338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CTGCTAGTAGAAAACCGAAA	50.0	40.0	57.8	53.0	1371-1493 bp	1.0	44.1
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	CTGCTAGTAGAAAACCGAAA	55.0	40.0	58.2	53.0	1366-1488 bp	1.0	44.1
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	CTGCTAGTAGAAAACCGAAA	50.0	40.0	58.4	53.0	1364-1486 bp	1.0	44.1
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	CTGCTAGTAGAAAACCGAAAT	50.0	38.1	57.8	53.7	1371-1493 bp	1.0	44.1
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	CTGCTAGTAGAAAACCGAAAT	55.0	38.1	58.2	53.7	1366-1488 bp	1.0	44.1