Hotspot

ycf4-cemA

LSC · 63919-64906 · 988 bp

Back to Combretum

Pi 0.0381

MarkerSeek score 65.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 23
pi: 0.0381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.61
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCGCGAGAAATTGAG	ATAGAGAGATCCACCAGGGC	50.0	55.0	58.0	58.0	807-1138 bp	1.0	85.7
ycf4-cemA_p2	TGAGAATTTGACTCCGCGAG	ATAGAGAGATCCACCAGGGC	50.0	55.0	58.0	58.0	815-1146 bp	1.0	85.7
ycf4-cemA_p3	TTAGCCTATTTCTTGCGCGT	ATAGAGAGATCCACCAGGGC	45.0	55.0	58.0	58.0	772-1103 bp	1.0	85.6
ycf4-cemA_p4	TGACTCCGCGAGAAATTGAG	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	802-1133 bp	1.0	85.3
ycf4-cemA_p5	TGAGAATTTGACTCCGCGAG	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	810-1141 bp	1.0	85.2