Hotspot

JXY33_pgt008-JXY33_pgt009

LSC · 32736-33176 · 441 bp

Back to Combretum

Pi 0.0664

MarkerSeek score 77.20

Primer available yes

Label JXY33_pgt008-JXY33_pgt009

Feature Metadata

label_name: JXY33_pgt008-JXY33_pgt009
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 28
pi: 0.0664
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
JXY33_pgt008-JXY33_pgt009_p1	ATTTGATTCCGTCGGGACTG	CTGGTTCAAATCCAGCTCGG	50.0	55.0	58.0	58.9	551-566 bp	1.0	79.3
JXY33_pgt008-JXY33_pgt009_p2	TTGGATTTGATTCCGTCGGG	CTGGTTCAAATCCAGCTCGG	50.0	55.0	58.3	58.9	555-570 bp	1.0	77.4
JXY33_pgt008-JXY33_pgt009_p3	GATTTGATTCCGTCGGGACT	CTGGTTCAAATCCAGCTCGG	50.0	55.0	57.7	58.9	552-567 bp	1.0	77.0
JXY33_pgt008-JXY33_pgt009_p4	ATTTGATTCCGTCGGGACTG	TGGTTCAAATCCAGCTCGG	50.0	52.6	58.0	57.8	550-565 bp	1.0	76.6
JXY33_pgt008-JXY33_pgt009_p5	CGGTGCTCTGACCAATTGAA	CTGGTTCAAATCCAGCTCGG	50.0	55.0	58.5	58.9	495-510 bp	1.0	76.4