Hotspot

psbM-JXY33_pgt008

LSC · 31865-32661 · 797 bp

Back to Combretum

Pi 0.0352

MarkerSeek score 72.60

Primer available yes

Label psbM-JXY33_pgt008

Feature Metadata

label_name: psbM-JXY33_pgt008
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 28
pi: 0.0352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-JXY33_pgt008_p1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	908-944 bp	1.0	56.2
psbM-JXY33_pgt008_p2	ACTAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.0	58.5	911-947 bp	1.0	54.7
psbM-JXY33_pgt008_p3	AGGAACTAGAATGAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	43.5	50.0	57.9	58.5	915-951 bp	1.0	54.1
psbM-JXY33_pgt008_p4	AGGAACTAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	56.8	58.5	915-951 bp	1.0	53.4
psbM-JXY33_pgt008_p5	GGAACTAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.2	58.5	914-950 bp	1.0	53.4