Hotspot

JXY33_pgt004-JXY33_pgt005

LSC · 8990-9690 · 701 bp

Back to Combretum

Pi 0.032

MarkerSeek score 75.10

Primer available yes

Label JXY33_pgt004-JXY33_pgt005

Feature Metadata

label_name: JXY33_pgt004-JXY33_pgt005
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 21
pi: 0.032
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
JXY33_pgt004-JXY33_pgt005_p1	CAATCCGACGCTTTAGTCCA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.0	57.6	814-820 bp	1.0	82.5
JXY33_pgt004-JXY33_pgt005_p2	AATCCGACGCTTTAGTCCAC	AAATCAAACCGAGGGATCCC	50.0	50.0	58.0	57.6	813-819 bp	1.0	82.5
JXY33_pgt004-JXY33_pgt005_p3	ACGGAAAGAGAGGGATTCGA	AAATCAAACCGAGGGATCCC	50.0	50.0	58.2	57.6	868-874 bp	1.0	81.2
JXY33_pgt004-JXY33_pgt005_p4	CAATCCGACGCTTTAGTCCA	CGGAAAAATCAAACCGAGGG	50.0	50.0	58.0	57.4	819-825 bp	1.0	81.0
JXY33_pgt004-JXY33_pgt005_p5	AATCCGACGCTTTAGTCCAC	CGGAAAAATCAAACCGAGGG	50.0	50.0	58.0	57.4	818-824 bp	1.0	81.0