Hotspot

ycf1

SSC · 121553-126865 · 5313 bp

Back to Comastoma

Pi 0.000753

MarkerSeek score 52.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5313
variable_sites: 4
pi: 0.000753
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGATAAACTGGGTGGATATGT	AATGGAGTGTTTCGCCTTGA	40.9	45.0	56.6	57.7	217 bp	1.0	51.2
ycf1_p2	TGGATAAACTGGGTGGATATGT	ATGGAGTGTTTCGCCTTGAA	40.9	45.0	56.6	57.7	216 bp	1.0	51.2
ycf1_p3	TGGATAAACTGGGTGGATATGT	GGAGTGTTTCGCCTTGAAGT	40.9	50.0	56.6	58.4	214 bp	1.0	50.1
ycf1_p4	TGGATAAACTGGGTGGATATGT	TGGAGTGTTTCGCCTTGAAG	40.9	50.0	56.6	58.4	215 bp	1.0	50.1
ycf1_p5	AAATGGATAAACTGGGTGGA	ATGGAGTGTTTCGCCTTGAA	40.0	45.0	54.2	57.7	219 bp	1.0	47.9