Hotspot

psbC

LSC · 32386-33807 · 1422 bp

Back to Comastoma

Pi 0.00141

MarkerSeek score 59.60

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 2
pi: 0.00141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GCAGCTGAAGATCCGGAATT	TGCCCTGATCCCAAGATGTA	50.0	50.0	58.3	58.1	1587 bp	1.0	76.6
psbC_p2	AGGTATTCGTGCTTGGATGG	TGCCCTGATCCCAAGATGTA	50.0	50.0	57.7	58.1	1531 bp	1.0	76.6
psbC_p3	GCAGCTGAAGATCCGGAATT	AGTATGCCCTGATCCCAAGA	50.0	50.0	58.3	57.8	1591 bp	1.0	75.9
psbC_p4	AGGTATTCGTGCTTGGATGG	AGTATGCCCTGATCCCAAGA	50.0	50.0	57.7	57.8	1535 bp	1.0	75.9
psbC_p5	TTCTCAAGAAATTCGCGCAG	TGCCCTGATCCCAAGATGTA	45.0	50.0	57.1	58.1	1603 bp	1.0	72.2