Hotspot

ndhD

SSC · 113126-114646 · 1521 bp

Back to Comastoma

Pi 0.00263

MarkerSeek score 55.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 4
pi: 0.00263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGTGTCGAATGAACGATTTTCA	AGGAGCACTGGTTTTTCTGG	36.4	50.0	56.7	58.0	1605 bp	1.0	53.4
ndhD_p2	AGTGTCGAATGAACGATTTTCA	ACCGACAAAATCAGTGCTCA	36.4	45.0	56.7	57.7	1635 bp	1.0	51.5
ndhD_p3	AGTGTCGAATGAACGATTTTCA	AGCACTGGTTTTTCTGGTCC	36.4	50.0	56.7	58.3	1602 bp	1.0	51.1
ndhD_p4	AGTGTCGAATGAACGATTTTCA	GGAGCACTGGTTTTTCTGGT	36.4	50.0	56.7	58.3	1604 bp	1.0	51.1
ndhD_p5	AGTGTCGAATGAACGATTTTCAA	AGGAGCACTGGTTTTTCTGG	34.8	50.0	57.3	58.0	1605 bp	1.0	50.8