Hotspot

trnL-CAA-ycf15

IRa · 141694-142099 · 406 bp

Back to Comastoma

Pi 0.00985
MarkerSeek score 60.40
Primer available yes
Label trnL-CAA-ycf15

Feature Metadata

label_name
trnL-CAA-ycf15
feature_type
igs
region
IRa
length_bp
406
variable_sites
4
pi
0.00985
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf15_p1 AAATGGTAGACACGCGAGAC AACAGCGAACAACCCATTCA 50.0 45.0 58.0 58.3 541 bp 1.0 78.0
trnL-CAA-ycf15_p2 AAATGGTAGACACGCGAGAC TGAACAGCGAACAACCCATT 50.0 45.0 58.0 58.3 543 bp 1.0 78.0
trnL-CAA-ycf15_p3 AAATGGTAGACACGCGAGAC TTGAACAGCGAACAACCCAT 50.0 45.0 58.0 58.3 544 bp 1.0 78.0
trnL-CAA-ycf15_p4 TGAAATGGTAGACACGCGAG AACAGCGAACAACCCATTCA 50.0 45.0 58.0 58.3 543 bp 1.0 78.0
trnL-CAA-ycf15_p5 TGAAATGGTAGACACGCGAG TGAACAGCGAACAACCCATT 50.0 45.0 58.0 58.3 545 bp 1.0 78.0