Hotspot

ndhB-trnL-CAA

IRa · 141051-141612 · 562 bp

Back to Comastoma

Pi 0

MarkerSeek score 34.90

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 562
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	GCAATTGCTCAGGATACGCT	GTCTCGCGTGTCTACCATTT	50.0	50.0	58.7	58.0	621 bp	1.0	74.4
ndhB-trnL-CAA_p2	GCAATTGCTCAGGATACGCT	CTCGCGTGTCTACCATTTCA	50.0	50.0	58.7	58.0	619 bp	1.0	74.4
ndhB-trnL-CAA_p3	GCAATTGCTCAGGATACGCT	TACCATTTCACCACCAAGGC	50.0	50.0	58.7	58.1	609 bp	1.0	73.8
ndhB-trnL-CAA_p4	GCAATTGCTCAGGATACGCT	GCGTGTCTACCATTTCACCA	50.0	50.0	58.7	58.2	616 bp	1.0	72.9
ndhB-trnL-CAA_p5	GCAATTGCTCAGGATACGCT	TCTCGCGTGTCTACCATTTC	50.0	50.0	58.7	57.7	620 bp	1.0	72.2