Hotspot

ndhF-rpl32

SSC · 109780-110546 · 767 bp

Back to Comastoma

Pi 0.00261

MarkerSeek score 60.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 767
variable_sites: 2
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACGGGAAGTGGAATGAA	AGCGGTCCAATATCCCTTCT	50.0	50.0	57.8	58.2	906-907 bp	1.0	77.2
ndhF-rpl32_p2	AACGGGAAGTGGAATGAAGG	AGCGGTCCAATATCCCTTCT	50.0	50.0	57.8	58.2	904-905 bp	1.0	77.1
ndhF-rpl32_p3	AGGAACGGGAAGTGGAATGA	AGCGGTCCAATATCCCTTCT	50.0	50.0	58.6	58.2	907-908 bp	1.0	73.6
ndhF-rpl32_p4	GGAACGGGAAGTGGAATGAA	AAGCGGTCCAATATCCCTTC	50.0	50.0	57.8	57.4	907-908 bp	1.0	73.6
ndhF-rpl32_p5	GGAACGGGAAGTGGAATGAA	GCGGTCCAATATCCCTTCTT	50.0	50.0	57.8	57.4	905-906 bp	1.0	73.6