Hotspot

rpl36-infA

LSC · 77841-77923 · 83 bp

Back to Comastoma

Pi 0.0241

MarkerSeek score 66.40

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 83
variable_sites: 2
pi: 0.0241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	ATAAGTCGTCCCCTCCTACG	TTTTGACTCCGCAACAAGGA	55.0	45.0	58.0	58.0	224 bp	1.0	80.2
rpl36-infA_p2	ATAAGTCGTCCCCTCCTACG	TGACTCCGCAACAAGGATTC	55.0	50.0	58.0	58.2	221 bp	1.0	79.0
rpl36-infA_p3	ATAAGTCGTCCCCTCCTACG	TTGACTCCGCAACAAGGATT	55.0	45.0	58.0	57.7	222 bp	1.0	78.4
rpl36-infA_p4	ATAAGTCGTCCCCTCCTACG	TTTGACTCCGCAACAAGGAT	55.0	45.0	58.0	57.7	223 bp	1.0	78.4
rpl36-infA_p5	ATAAGTCGTCCCCTCCTACG	TAAATGGTTTGAACGCCCCT	55.0	45.0	58.0	57.7	194 bp	1.0	78.3