Hotspot

trnT-GGU-psbD

LSC · 30234-31376 · 1143 bp

Back to Comastoma

Pi 0.00264

MarkerSeek score 60.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 3
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1278-1285 bp	1.0	80.1
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1281-1288 bp	1.0	79.9
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	GACCAGCCTACAAACACGAA	55.0	50.0	58.1	58.1	1295-1302 bp	1.0	79.1
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	AAACACGAAACGGTCCCTAC	55.0	50.0	58.1	58.1	1284-1291 bp	1.0	79.1
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAAACACGAAACGGTCCCTA	55.0	50.0	58.1	58.1	1285-1292 bp	1.0	79.1