Hotspot

trnY-GUA-trnE-UUC

LSC · 29269-29332 · 64 bp

Back to Comastoma

Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TGTCGAATTATTGGGCCGAG	TAGCGGTTATGCAAGTAGCC	50.0	50.0	58.1	57.8	249 bp	1.0	78.0
trnY-GUA-trnE-UUC_p2	TGTCGAATTATTGGGCCGAG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	58.1	58.3	204 bp	1.0	77.9
trnY-GUA-trnE-UUC_p3	TGTCGAATTATTGGGCCGAG	TCGTCTAGTGGTTCAGGACA	50.0	50.0	58.1	57.7	225 bp	1.0	77.7
trnY-GUA-trnE-UUC_p4	TGTCGAATTATTGGGCCGAG	AAGTAGCCCCCATCGTCTAG	50.0	55.0	58.1	58.3	237 bp	1.0	77.6
trnY-GUA-trnE-UUC_p5	TGTCGAATTATTGGGCCGAG	GTTATGCAAGTAGCCCCCAT	50.0	50.0	58.1	57.6	244 bp	1.0	77.0