Hotspot

rpoB-trnC-GCA

LSC · 25492-26776 · 1285 bp

Back to Comastoma

Pi 0.00156

MarkerSeek score 59.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 2
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCGTCAAACCCTGATCAAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1445 bp	1.0	78.0
rpoB-trnC-GCA_p2	TCCGTCAAACCCTGATCAAC	TATCAGCCGAAGAGGGGATT	50.0	50.0	57.8	57.9	1491 bp	1.0	77.9
rpoB-trnC-GCA_p3	TCCGTCAAACCCTGATCAAC	TTATCAGCCGAAGAGGGGAT	50.0	50.0	57.8	57.9	1492 bp	1.0	77.9
rpoB-trnC-GCA_p4	CGTCAAACCCTGATCAACGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1443 bp	1.0	77.8
rpoB-trnC-GCA_p5	CGTCAAACCCTGATCAACGA	TATCAGCCGAAGAGGGGATT	50.0	50.0	58.2	57.9	1489 bp	1.0	77.6