Hotspot

trnH-GUG-psbA

LSC · 74-419 · 346 bp

Back to Comastoma

Pi 0.0491

MarkerSeek score 81.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 17
pi: 0.0491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCGGGCTAATCTTGGTATGG	50.0	55.0	57.9	57.8	471 bp	1.0	80.3
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCGGGCTAATCTTGGTATGG	50.0	55.0	58.2	57.8	472 bp	1.0	80.1
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	CCGGGCTAATCTTGGTATGG	50.0	55.0	57.7	57.8	464 bp	1.0	78.8
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	ACCGGGCTAATCTTGGTATG	50.0	50.0	57.9	57.1	472 bp	1.0	74.4
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	ACCGGGCTAATCTTGGTATG	50.0	50.0	58.2	57.1	473 bp	1.0	74.1