Hotspot

ndhF

SSC · 111434-113683 · 2250 bp

Back to Coluria

Pi 0.0213

MarkerSeek score 58.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 48
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGTCCGTTACGCTATATAAA	AGAACCGGTGAATTAGAAACA	40.0	38.1	52.8	55.2	2335-2663 bp	1.0	41.5
ndhF_p2	CGTCCGTTACGCTATATAAAA	AGAACCGGTGAATTAGAAACA	38.1	38.1	53.7	55.2	2335-2663 bp	1.0	41.5
ndhF_p3	CGTCCGTTACGCTATATAAA	AAGAACCGGTGAATTAGAAACA	40.0	36.4	52.8	55.9	2336-2664 bp	1.0	41.5
ndhF_p4	CGTCCGTTACGCTATATAAA	AGAACCGGTGAATTAGAAACAA	40.0	36.4	52.8	55.9	2335-2663 bp	1.0	41.5
ndhF_p5	CGTCCGTTACGCTATATAAAAA	AGAACCGGTGAATTAGAAACA	36.4	38.1	54.5	55.2	2335-2663 bp	1.0	41.5