Hotspot

ndhE-ndhG

SSC · 119359-119592 · 234 bp

Back to Coluria

Pi 0.0315

MarkerSeek score 75.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 234
variable_sites: 7
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTTCGGCTCGTGATCAATCC	TGATAGGCGCAATTGCTGTA	50.0	45.0	58.3	57.7	331-343 bp	1.0	79.1
ndhE-ndhG_p2	TTTCGGCTCGTGATCAATCC	TAGTTGCTTTGATAGGCGCA	50.0	45.0	58.3	57.6	340-352 bp	1.0	78.4
ndhE-ndhG_p3	CGGCTCGTGATCAATCCATA	TGATAGGCGCAATTGCTGTA	50.0	45.0	57.6	57.7	328-340 bp	1.0	78.4
ndhE-ndhG_p4	TTTCGGCTCGTGATCAATCC	AGTTGCTTTGATAGGCGCAA	50.0	45.0	58.3	58.5	339-351 bp	1.0	77.9
ndhE-ndhG_p5	CGGCTCGTGATCAATCCATA	TAGTTGCTTTGATAGGCGCA	50.0	45.0	57.6	57.6	337-349 bp	1.0	77.8