Hotspot

rpl32-trnL-UAG

SSC · 114946-115520 · 575 bp

Back to Coluria

Pi 0.0355

MarkerSeek score 72.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 575
variable_sites: 20
pi: 0.0355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCACTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	686-695 bp	1.0	81.7
rpl32-trnL-UAG_p2	GGCAGCACTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	687-696 bp	1.0	81.7
rpl32-trnL-UAG_p3	GGCAGCACTGAAAGCTTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	696-705 bp	1.0	80.7
rpl32-trnL-UAG_p4	GGCAGCACTGAAAGCTTTTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.8	57.8	695-704 bp	1.0	80.1
rpl32-trnL-UAG_p5	GGCAGCACTGAAAGCTTTTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.8	57.8	694-703 bp	1.0	80.1