Hotspot

ndhF-rpl32

SSC · 113684-114786 · 1103 bp

Back to Coluria

Pi 0.046

MarkerSeek score 79.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1103
variable_sites: 48
pi: 0.046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAACGGT	TGAACTAGAGGTGCGCTTTT	50.0	45.0	57.8	57.5	1166-1188 bp	1.0	81.4
ndhF-rpl32_p2	CTGGAAGTGGAATGAACGGT	TTGAACTAGAGGTGCGCTTT	50.0	45.0	57.8	57.5	1167-1189 bp	1.0	81.4
ndhF-rpl32_p3	CTGGAAGTGGAATGAACGGT	TTTGAACTAGAGGTGCGCTT	50.0	45.0	57.8	57.5	1168-1190 bp	1.0	81.4
ndhF-rpl32_p4	CTGGAAGTGGAATGAACGGT	TTTTGAACTAGAGGTGCGCT	50.0	45.0	57.8	57.5	1169-1191 bp	1.0	81.4
ndhF-rpl32_p5	CTGGAAGTGGAATGAACGGT	TGAACTAGAGGTGCGCTTTTT	50.0	42.9	57.8	58.2	1166-1188 bp	1.0	76.6