Hotspot

psaJ-rpl33

LSC · 67802-68272 · 471 bp

Back to Coluria

Pi 0.0424

MarkerSeek score 72.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 19
pi: 0.0424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	507-530 bp	1.0	83.3
psaJ-rpl33_p2	GTTCGGGTCTTTAGCAGGTT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	552-575 bp	1.0	82.6
psaJ-rpl33_p3	TGGCACCGGTAGTAAGTACT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	577-600 bp	1.0	82.1
psaJ-rpl33_p4	GTGGCACCGGTAGTAAGTAC	GGACATCTTTACCCTTGGCC	55.0	55.0	57.7	58.2	578-601 bp	1.0	81.5
psaJ-rpl33_p5	ATGGTTCGGGTCTTTAGCAG	GGACATCTTTACCCTTGGCC	50.0	55.0	57.6	58.2	555-578 bp	1.0	80.8