Hotspot

petA-psbJ

LSC · 63289-64376 · 1088 bp

Back to Coluria

Pi 0.0411

MarkerSeek score 70.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1088
variable_sites: 44
pi: 0.0411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCAATTGTCCGAAATGAATTTCT	TGGGTCATATTCTGGATTAGGT	30.4	40.9	55.1	56.0	1147-1235 bp	1.0	44.2
petA-psbJ_p2	TCAATTGTCCGAAATGAATTTCT	GGGTCATATTCTGGATTAGGTTCA	30.4	41.7	55.1	57.8	1146-1234 bp	1.0	44.2
petA-psbJ_p3	TCAATTGTCCGAAATGAATTTCT	TGGGTCATATTCTGGATTAGGTTC	30.4	41.7	55.1	57.8	1147-1235 bp	1.0	44.2
petA-psbJ_p4	TCAATTGTCCGAAATGAATTTCT	TGGGTCATATTCTGGATTAGGTT	30.4	39.1	55.1	56.7	1147-1235 bp	1.0	44.2
petA-psbJ_p5	TTCAATTGTCCGAAATGAATTTCT	TGGGTCATATTCTGGATTAGGT	29.2	40.9	55.9	56.0	1148-1236 bp	1.0	44.2