Hotspot

accD-psaI

LSC · 59365-59808 · 444 bp

Back to Coluria

Pi 0.0389

MarkerSeek score 69.10

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 444
variable_sites: 17
pi: 0.0389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAGGGCGTTCTGAGTGAAT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	578-645 bp	1.0	78.4
accD-psaI_p2	AAGGGCGTTCTGAGTGAATT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	577-644 bp	1.0	78.4
accD-psaI_p3	AAAGGGCGTTCTGAGTGAAT	TTGCCGGAAATACTAAGCCC	45.0	50.0	57.4	58.0	569-636 bp	1.0	78.3
accD-psaI_p4	AAGGGCGTTCTGAGTGAATT	TTGCCGGAAATACTAAGCCC	45.0	50.0	57.4	58.0	568-635 bp	1.0	78.3
accD-psaI_p5	AAGGGCGTTCTGAGTGAATT	AGAAACCATTGCAATTGCCG	45.0	45.0	57.4	57.9	582-649 bp	1.0	77.9