Hotspot

ycf3-trnS-GGA

LSC · 45317-45936 · 620 bp

Back to Coluria

Pi 0.0201

MarkerSeek score 70.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 12
pi: 0.0201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	788-804 bp	1.0	69.7
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	45.0	50.0	56.4	58.2	789-805 bp	1.0	69.7
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	738-754 bp	1.0	69.6
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTCAA	45.0	45.0	56.4	57.8	739-755 bp	1.0	69.6
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	AGTTCCAATGCTACGCCTTC	45.0	50.0	56.4	58.3	740-756 bp	1.0	69.1