Hotspot

psbM-trnD-GUC

LSC · 29758-30760 · 1003 bp

Back to Coluria

Pi 0.0305
MarkerSeek score 65.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1003
variable_sites
30
pi
0.0305
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 35.0 50.0 52.0 58.5 1088-1098 bp 1.0 43.7
psbM-trnD-GUC_p2 ATGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 33.3 50.0 52.7 58.5 1089-1099 bp 1.0 43.7
psbM-trnD-GUC_p3 TGCGAGAATATTTACTTCCAT TTCAATTGGTCAGAGCACCG 33.3 50.0 52.7 58.5 1088-1098 bp 1.0 43.7
psbM-trnD-GUC_p4 AATGCGAGAATATTTACTTCCA TTCAATTGGTCAGAGCACCG 31.8 50.0 53.6 58.5 1090-1100 bp 1.0 43.7
psbM-trnD-GUC_p5 TGCGAGAATATTTACTTCCA GTTCAATTGGTCAGAGCACC 35.0 50.0 52.0 57.0 1089-1099 bp 1.0 43.7