Hotspot

trnR-UCU-atpA

LSC · 10259-10859 · 601 bp

Back to Coluria

Pi 0.0461

MarkerSeek score 77.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 27
pi: 0.0461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAAGGAGTGAAAAGCGTCCA	CAGGACCAGATGGAACGTTT	45.0	50.0	57.7	57.8	728-803 bp	1.0	80.2
trnR-UCU-atpA_p2	AAGGAGTGAAAAGCGTCCAT	CAGGACCAGATGGAACGTTT	45.0	50.0	57.4	57.8	727-802 bp	1.0	78.4
trnR-UCU-atpA_p3	AGGAGTGAAAAGCGTCCATT	CAGGACCAGATGGAACGTTT	45.0	50.0	57.4	57.8	726-801 bp	1.0	78.4
trnR-UCU-atpA_p4	GGAGTGAAAAGCGTCCATTG	CAGGACCAGATGGAACGTTT	50.0	50.0	57.4	57.8	725-800 bp	1.0	78.0
trnR-UCU-atpA_p5	AAAGGAGTGAAAAGCGTCCA	TCAGGACCAGATGGAACGTT	45.0	50.0	57.7	58.7	729-804 bp	1.0	76.4