Hotspot

trnG-UCC-trnR-UCU

LSC · 10016-10186 · 171 bp

Back to Coluria

Pi 0.037

MarkerSeek score 74.20

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 6
pi: 0.037
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGCAAATAAACTGCGTCCAA	50.0	40.0	58.0	56.5	289-298 bp	1.0	72.2
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGCAAATAAACTGCGTCCAA	55.0	40.0	58.1	56.5	292-301 bp	1.0	72.0
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	ATGCAAATAAACTGCGTCCA	50.0	40.0	58.0	56.3	290-299 bp	1.0	70.4
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	ATGCAAATAAACTGCGTCCA	55.0	40.0	58.1	56.3	293-302 bp	1.0	70.1
trnG-UCC-trnR-UCU_p5	CCCTAGCCTTCCAAGCTAAC	TGCAAATAAACTGCGTCCAA	55.0	40.0	57.7	56.5	293-302 bp	1.0	69.7