Hotspot

psbC

LSC · 34118-35539 · 1422 bp

Back to Colquhounia

Pi 0.000844

MarkerSeek score 48.10

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 3
pi: 0.000844
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GTTTCTCAGGAAATTCGCGC	GCATTGGATCTCCTGTCTCA	50.0	50.0	58.1	57.0	1556 bp	1.0	71.3
psbC_p2	CGTTTCTCAGGAAATTCGCG	GCATTGGATCTCCTGTCTCA	50.0	50.0	57.9	57.0	1557 bp	1.0	71.0
psbC_p3	AGGTATTCGTGCTTGGATGG	GCATTGGATCTCCTGTCTCA	50.0	50.0	57.7	57.0	1482 bp	1.0	69.5
psbC_p4	GTTTCTCAGGAAATTCGCGC	CAAGCATTGGATCTCCTGTCT	50.0	47.6	58.1	57.7	1559 bp	1.0	69.0
psbC_p5	GTTTCTCAGGAAATTCGCGC	AGCATTGGATCTCCTGTCTC	50.0	50.0	58.1	56.7	1557 bp	1.0	68.8