Hotspot

petB

LSC · 74108-75487 · 1380 bp

Back to Colquhounia

Pi 0.00478

MarkerSeek score 51.10

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1380
variable_sites: 16
pi: 0.00478
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCACAAAGTCCTGGCTTTTGA	TCCTATTATTCCTCCTCTAAGTGA	42.9	37.5	58.0	55.6	1560-1561 bp	1.0	40.8
petB_p2	TCACAAAGTCCTGGCTTTTGA	TCCTCCTCTAAGTGATAAATGATTGA	42.9	34.6	58.0	57.4	1551-1552 bp	1.0	40.8
petB_p3	TCACAAAGTCCTGGCTTTTGA	TCCTCCTCTAAGTGATAAATGA	42.9	36.4	58.0	53.4	1551-1552 bp	1.0	40.8
petB_p4	AGAATCACAAAGTCCTGGCT	TCCTATTATTCCTCCTCTAAGTGA	45.0	37.5	56.7	55.6	1564-1565 bp	1.0	40.8
petB_p5	TCACAAAGTCCTGGCTTTTGA	TCCATACTTGTAGCAATAAAATCCT	42.9	32.0	58.0	56.3	1581-1582 bp	1.0	40.8