Hotspot

ndhC-trnV(UAC)

LSC · 50378-51321 · 944 bp

Back to Colquhounia

Pi 0.0206

MarkerSeek score 50.40

Primer available yes

Label ndhC-trnV(UAC)

Feature Metadata

label_name: ndhC-trnV(UAC)
feature_type: igs
region: LSC
length_bp: 944
variable_sites: 46
pi: 0.0206
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV(UAC)_p1	CTAGAAAGTTTCTCCGGCCC	CGATCTAGACCAGAGGCAGA	55.0	55.0	58.0	58.0	1367-1376 bp	1.0	83.3
ndhC-trnV(UAC)_p2	GGGGCTAAAATCCCGGAAAT	CGATCTAGACCAGAGGCAGA	50.0	55.0	57.9	58.0	1337-1346 bp	1.0	82.9
ndhC-trnV(UAC)_p3	CTAGAAAGTTTCTCCGGCCC	ATCTAGACCAGAGGCAGACC	55.0	55.0	58.0	57.9	1365-1374 bp	1.0	82.8
ndhC-trnV(UAC)_p4	AGGGGGCCATTTTCATTGTT	CGATCTAGACCAGAGGCAGA	45.0	55.0	58.0	58.0	1917-1926 bp	1.0	82.1
ndhC-trnV(UAC)_p5	CTAAGGAGGGGGCCATTTTC	CGATCTAGACCAGAGGCAGA	55.0	55.0	57.9	58.0	1923-1932 bp	1.0	81.8