Hotspot

trnF(GAA)-ndhJ

LSC · 48043-48706 · 664 bp

Back to Colquhounia

Pi 0.00301

MarkerSeek score 49.80

Primer available yes

Label trnF(GAA)-ndhJ

Feature Metadata

label_name: trnF(GAA)-ndhJ
feature_type: igs
region: LSC
length_bp: 664
variable_sites: 5
pi: 0.00301
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(GAA)-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	806 bp	1.0	79.8
trnF(GAA)-ndhJ_p2	CTACATTGGGAATGGTCGGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	823 bp	1.0	79.5
trnF(GAA)-ndhJ_p3	TGCTACATTGGGAATGGTCG	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	825 bp	1.0	79.5
trnF(GAA)-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	770 bp	1.0	79.4
trnF(GAA)-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GATAATCATCCGCGCCTGAA	55.0	50.0	58.0	58.2	845 bp	1.0	78.8