Hotspot

rps16-trnQ(UUG)

LSC · 6218-7375 · 1158 bp

Back to Colquhounia

Pi 0.0244

MarkerSeek score 70.70

Primer available yes

Label rps16-trnQ(UUG)

Feature Metadata

label_name: rps16-trnQ(UUG)
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 66
pi: 0.0244
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ(UUG)_p1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.9	58.0	1113-1320 bp	1.0	87.4
rps16-trnQ(UUG)_p2	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1055-1262 bp	1.0	87.1
rps16-trnQ(UUG)_p3	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1096-1303 bp	1.0	87.0
rps16-trnQ(UUG)_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1038-1245 bp	1.0	86.7
rps16-trnQ(UUG)_p5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1114-1321 bp	1.0	86.4