Hotspot

rps15

SSC · 129953-130231 · 279 bp

Back to Colocasia

Pi 0.00179

MarkerSeek score 25.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 279
variable_sites: 1
pi: 0.00179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TCCCAACGCGTTACATAAGG	TCGGCGAAATCCTCTTAGGT	50.0	50.0	58.0	58.5	834-842 bp	1.0	77.8
rps15_p2	AACACCATGCATTGATGGGT	TCGGCGAAATCCTCTTAGGT	45.0	50.0	58.1	58.5	697-705 bp	1.0	77.8
rps15_p3	TGCTTCCTCTCCCCCAATAA	TCGGCGAAATCCTCTTAGGT	50.0	50.0	58.0	58.5	1264-1272 bp	1.0	77.1
rps15_p4	TTGCTTCCTCTCCCCCAATA	TCGGCGAAATCCTCTTAGGT	50.0	50.0	58.0	58.5	1265-1273 bp	1.0	77.1
rps15_p5	TCTGTGCACCAATATCTGCC	TCGGCGAAATCCTCTTAGGT	50.0	50.0	58.0	58.5	1008 bp	0.25	45.5