Hotspot

ndhD

SSC · 121449-122951 · 1503 bp

Back to Colocasia

Pi 0.00166

MarkerSeek score 47.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 5
pi: 0.00166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACAATTCCAACTCACTTCCCA	CGGGTTTTTCTGGTCAAAGC	42.9	50.0	57.4	57.9	1588 bp	1.0	66.4
ndhD_p2	ACAATTCCAACTCACTTCCCA	GGGTTTTTCTGGTCAAAGCG	42.9	50.0	57.4	57.9	1587 bp	1.0	66.4
ndhD_p3	ACAATTCCAACTCACTTCCCAT	CGGGTTTTTCTGGTCAAAGC	40.9	50.0	57.9	57.9	1588 bp	1.0	62.2
ndhD_p4	ACAATTCCAACTCACTTCCCAT	GGGTTTTTCTGGTCAAAGCG	40.9	50.0	57.9	57.9	1587 bp	1.0	62.2
ndhD_p5	ACAATTCCAACTCACTTCCCA	GGTTTTTCTGGTCAAAGCGT	42.9	45.0	57.4	57.1	1586 bp	1.0	60.6