Hotspot

ndhE-ndhG

SSC · 124607-124968 · 362 bp

Back to Colocasia

Pi 0.00276

MarkerSeek score 61.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 362
variable_sites: 2
pi: 0.00276
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GTGCTCTAACCATGCTTCGA	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	475-494 bp	1.0	79.6
ndhE-ndhG_p2	TGCTCTAACCATGCTTCGAC	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	474-493 bp	1.0	79.6
ndhE-ndhG_p3	AGTGCTCTAACCATGCTTCG	AGTTGCCTTGATAGGTGCAA	50.0	45.0	58.0	57.4	476-495 bp	1.0	79.5
ndhE-ndhG_p4	AACCATGCTTCGACTTGTGA	AGTTGCCTTGATAGGTGCAA	45.0	45.0	57.7	57.4	468-487 bp	1.0	77.6
ndhE-ndhG_p5	GCTCTAACCATGCTTCGACT	AGTTGCCTTGATAGGTGCAA	50.0	45.0	57.7	57.4	473-492 bp	1.0	77.2