Hotspot

tRNA-Asn(GUU)-ndhF

SSC · 114886-115954 · 1069 bp

Back to Colocasia

Pi 0.014

MarkerSeek score 58.70

Primer available yes

Label tRNA-Asn(GUU)-ndhF

Feature Metadata

label_name: tRNA-Asn(GUU)-ndhF
feature_type: igs
region: SSC
length_bp: 1069
variable_sites: 23
pi: 0.014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Asn(GUU)-ndhF_p1	AGGATTCGAACCTACGACCA	TATTTAGTAGGGGGCGGACG	50.0	55.0	58.2	58.7	997-1270 bp	1.0	79.0
tRNA-Asn(GUU)-ndhF_p2	AATCAGTTAACAGCCGACCG	TATTTAGTAGGGGGCGGACG	50.0	55.0	58.3	58.7	978-1251 bp	1.0	78.1
tRNA-Asn(GUU)-ndhF_p3	CCCCAAGTAGGATTCGAACC	TATTTAGTAGGGGGCGGACG	55.0	55.0	57.7	58.7	1005-1278 bp	1.0	77.6
tRNA-Asn(GUU)-ndhF_p4	CGAACCTACGACCAATCAGT	TATTTAGTAGGGGGCGGACG	50.0	55.0	57.4	58.7	991-1264 bp	1.0	75.1
tRNA-Asn(GUU)-ndhF_p5	GGATTCGAACCTACGACCAA	TATTTAGTAGGGGGCGGACG	50.0	55.0	57.3	58.7	996-1269 bp	1.0	75.0