Hotspot

psbE-petL

LSC · 69800-71028 · 1229 bp

Back to Colocasia

Pi 0.00249

MarkerSeek score 62.60

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 6
pi: 0.00249
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACGAATAACCAACCTGCA	GCGCTGGGGTTATAGTTGAA	45.0	50.0	57.4	58.0	1390-1409 bp	1.0	77.3
psbE-petL_p2	TGACGAATAACCAACCTGCA	AAGCGCTGGGGTTATAGTTG	45.0	50.0	57.4	58.0	1392-1411 bp	1.0	77.2
psbE-petL_p3	TGACGAATAACCAACCTGCA	AATCAAAAGCGCTGGGGTTA	45.0	45.0	57.4	58.1	1398-1417 bp	1.0	76.9
psbE-petL_p4	CAAAAGAACGTTCTCCCGTG	GCGCTGGGGTTATAGTTGAA	50.0	50.0	57.3	58.0	1306-1325 bp	1.0	76.5
psbE-petL_p5	TGACGAATAACCAACCTGCA	ATCAAAAGCGCTGGGGTTAT	45.0	45.0	57.4	57.9	1397-1416 bp	1.0	76.4